Journal: Molecular cell

Article Title: Two-parameter mobility assessments discriminate diverse regulatory factor behaviors in chromatin

doi: 10.1016/j.molcel.2020.05.036

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: TETO-FUW-HNF1A/TETO-FUW-HNF4A/TETO-FUW-MYC/TETO-FUW-GATA4/TETO-FUW-OCT4/TETO-FUW-KLF4/TETO-FUW-SOX2/TETO-FUW-PU.1/TETO-FUW-FOXA1EKQAAA ORF of interest (see ) from the corresponding pCMV-ORF-HaloTag were PCR amplified with the adequate primers (see Table S3 , JL27 to JL35) and assembled using Gibson Assembly® Master Mix kit (NEB E2611L) with EcoRI (NEB) digested TETO-FUW-OCT4 (Addgene plasmid #20323). table ft1 table-wrap mode="anchored" t5 caption a7 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-FOXA1 ABCAM 55178 Mouse anti-POL2 COVANCE 8WG16 Bacterial and Virus Strains pENTR4/HALO Gift from Eric Campeau Addgene W876-1 H2B-Cherry Gift from Robert Benezra Addgene 20972 pmWasabi-CT Allele Biotech ABP-FP-WCNCSP Tet-O-FUW-OCT4 Gift from Rudolf Jaenisch Addgene 20323 FOXA1-HALO This paper FOXA1-HALO FOXA1-NHAA-HALO This paper FOXA1-NHAA-HALO FOXA1-RRAA-HALO This paper FOXA1-RRAA-HALO FOXA1-EKQAAA-HALO This paper FOXA1-EKQAAA-HALO GATA4-HALO This paper GATA4-HALO HNF1A-HALO This paper HNF1A-HALO HNF4A-HALO This paper HNF4A-HALO cMYC-HALO This paper cMYC-HALO SOX2-HALO This paper SOX2-HALO OCT4-HALO This paper OCT4-HALO KLF4-HALO This paper KLF4-HALO PU.1-HALO This paper PU.1-HALO SUv39h1-HALO This paper SUv39h1-HALO SUv39h2-HALO This paper SUv39h2-HALO HP1α-HALO This paper HP1α-HALO HP1β-HALO This paper HP1β-HALO HP1γ-HALO This paper HP1γ-HALO Biological Samples Chemicals, Peptides, and Recombinant Proteins Critical Commercial Assays MinElute PCR purification kit QIAGEN 28004 MiniPrep QIAGEN 27106 MAXIPREP QIAGEN 12263 Gel extraction kit QIAGEN 28706 Gibson Assembly® kit NEB E5510 T4 DNA Ligase high concentration NEB M0202T Deposited Data Experimental Models: Cell Lines DH5A competent cells Invitrogen 18265017 H2.35 Lab stock H2.35 H2.35 FOXA1 This paper H2.35_FOXA1-HALO H2.35 FOXA1-NHAA This paper H2.35_NHAA-HALO H2.35 FOXA1-RRAA This paper H2.35_RRAA-HALO Experimental Models: Organisms/Strains Oligonucleotides Table S3 This paper Table S3 Recombinant DNA Software and Algorithms Serial Cloner SerialBasics Serial Cloner 2.6 PRISM 7 GraphPad https://www.graphpad.com/scientific-software/prism/ Fiji is Just ImageJ (Fiji) version 2.0.0-rc/1.51f ImageJ https://imagej.net/ Oni acquisition software Oxford Nanoimaging https://oni.bio MATLAB Mathworks www.mathworks.com Microsoft Office Microsoft www.office.com MATLAB script - track classification MATLAB script SMT_Motion_Classifier.m MATLAB script - MSD comparison MATLAB script Compare_MSD_Results Other Oni Nanoimager S (HiLO microscopy) Oxford Nanoimaging https://oni.bio Open in a separate window KEY RESOURCES TABLE

Techniques: Virus, Recombinant, Purification, Gel Extraction, Concentration Assay, Software, Comparison, Microscopy